https://hdl.handle.net/20.500.14178/1 Faculties Mon, 27 Jul 2026 06:57:34 GMT 2026-07-27T06:57:34Z https://hdl.handle.net/20.500.14178/3883 Exploring the full spectrum of reproductive experiences Slabá, Jitka; Jírová, Jitka; Šťastná, Anna; Kocourková, Jiřina Objective: The study examines changes across cohorts in terms of the timing and frequency of the full spectrum of reproductive events and explores whether these reproductive trajectories reflect broader trends towards the destandardisation of family formation. Furthermore, the study assesses the quality and limitations of Czech health registry data by comparing them with official vital statistics. Background: Analysing fertility alone is insufficient with respect to understanding individual reproductive trajectories since reproductive experiences are interconnected across the lifespan and may be influenced by other reproductive events that affect both the tempo and the quantum - especially within the context of fertility postponement.Method: This study employs individual data obtained from the Czech National Health Information System (NHIS) that enable the holistic analysis of reproductive trajectories including live births, induced abortions, in vitro fertilisation, miscarriages and stillbirths across selected cohorts of women (1976-1991).Results: The younger cohorts experienced delayed and less frequent reproductive events. However, the lower incidence of adverse events partly reflected fewer conceptions due to the postponement of reproduction to older ages. The most common combination of event types involved live births with induced abortions or miscarriages. Reproductive trajectories were found to diversify with age but exhibited limited variation across the cohorts, and an early live birth remained linked to higher completed fertility. The NHIS data were largely in line with the vital statistics, with the moderate underestimation of live births and the under-reporting of early miscarriages and induced abortions.Conclusion: The findings highlight the potential and limitations of health registry data in terms of capturing the full spectrum of reproductive events. Forming a deeper understanding of women's reproductive trajectories and experiences, enabled via the use of linked health register data, is essential in terms of developing policies on reproductive health and rights in the context of declining fertility levels and the postponement of childbearing to later ages. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/20.500.14178/3883 2026-01-01T00:00:00Z https://hdl.handle.net/20.500.14178/3882 Analysis of triggering factors behind the October 2023 South Lhonak GLOF event in the Sikkim Himalaya using multiple remote sensing data Vilímek, Vít; Kropáček, Jan; Chowdhury, Arindam; Baťka, Jan; De, Sunil Kumar A catastrophic Glacial Lake Outburst Flood (GLOF) occurred from the South Lhonak Glacial Lake (SLGL) in the Sikkim Himalaya between October 3 and 4, 2023, highlighting the urgent need to understand its triggering mechanisms. Given the inaccessibility of the lake region, we employed multiple state-of-the-art remote sensing approaches, including optical image interpretation, interferometric coherence and geospatial analysis, to examine all plausible triggering mechanisms while systematically eliminating non-contributing factors. Our primary objective was to evaluate the potential triggers, with particular focus on a landslide originating from the left lateral moraine, which appears to have been the direct cause of the GLOF. The estimated landslide volume was similar to 13.1 +/- 2.7 x 10(6) m(3). Subsequent analysis aimed to determine the underlying cause of this landslide. Results indicate that increased inflow-comprising both surface runoff and subsurface seepage, into the left lateral moraine significantly elevated slope saturation, thereby compromising stability and triggering the failure. Other possible contributors were also investigated but found to have negligible or no influence on the event. These included hydrostatic pressure from the adjacent North Lhonak Glacier, a potential outburst from the North Lhonak Glacial Lake (NLGL), blockage of the SLGL outlet by landslide, seismic activity, and precipitation events. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/20.500.14178/3882 2026-01-01T00:00:00Z https://hdl.handle.net/20.500.14178/3881 Preventing Radicalisation, or Forcing Ideological Consent? Crises, Uncertainty Reduction and Collective Identity Negotiation in Collaborative Communities of Online Third Spaces Vochocová, Lenka; Rosenfeldová, Jana With regard to the recent accumulation of crisis events in Europe, scholars stress the potential of online spaces to reduce people's uncertainty through collective identity formation, providing their members with emotional support and recognition. However, polarisation and radicalisation are mentioned as possible negative consequences of online community formation in crisis times. "Third spaces" are described as online forums where political talk emerges against the background of the primary purpose of the environment - exchanging ideas or goods and mutual support. Our article explores how the "purpose-based" collective identity of the participants of a parenting forum, a typical "third space," is challenged with their potentially conflicting political identities in the context of strongly polarising issues such as the COVID-19 pandemic and the Russian-Ukrainian conflict. A qualitative analysis of nearly 2,500 contributions confirms that, to a certain extent, a third space constitutes an environment of cooperation and support even during major societal crises and provides space to negotiate conflicting ideological identities. However, broader societal polarisation is reflected in the forum which clearly sets boundaries to diversity, strictly excluding opinions considered extreme or unacceptable by the majority. Proponents of minority opinions are thus pushed to the margins, where they may radicalise. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/20.500.14178/3881 2026-01-01T00:00:00Z https://hdl.handle.net/20.500.14178/3880 PRPF8-associated retinitis pigmentosa variant induces human neural retina-autonomous photoreceptor defects Zimmann, Felix; Banik, Poulami; Kubovčiak, Jan; Delattre, Mathys; Thakur, Prasoon K.; Čapek, Martin; Kolář, Michal; Hrubá, Eva; Dobrovolný, Robert; Cvačková, Zuzana; Bárta, Tomáš; Staněk, David Retinitis pigmentosa (RP) is an inherited retinal disorder characterized by the progressive loss of photoreceptors that currently lacks effective treatment. Here, we investigated the effects of the PRPF8-Y2334N variant on neural retina cells using human induced pluripotent stem cell (hiPSC)-derived retinal organoids. Expression of PRPF8-Y2334N variant resulted in photoreceptor defects, including thinning of the outer segment layer. This indicates that the neural retina is impacted independently of retinal pigment epithelium (RPE). At the molecular level, we observed relatively minor changes in mRNA expression in multiple retinal cells. We also found splicing alterations in genes associated with neural and retinal diseases, including those involved in intraflagellar transport, suggesting that these genes may represent common targets of splicing factor mutations. Finally, we detected the misexpression of several circular RNAs (circRNAs), which could serve as early biomarkers of splicing defects caused by RP mutations. Together, we present a model of RP that recapitulates photoreceptor degeneration and demonstrates that these defects are independent of RPE degeneration. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/20.500.14178/3880 2026-01-01T00:00:00Z