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Proteogenomic Characterization of Pseudomonas veronii SM-20 Growing on Phenanthrene as Only Carbon and Energy Source

dc.contributor.authorZavala Meneses, Sofia Guadalupe
dc.contributor.authorFirrincieli, Andrea
dc.contributor.authorChalova, Petra
dc.contributor.authorPajer, Petr
dc.contributor.authorCheccucci, Alice
dc.contributor.authorŠkultéty, Ľudovít
dc.contributor.authorCappelletti, Martina
dc.date.accessioned2024-10-07T08:46:07Z
dc.date.available2024-10-07T08:46:07Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2646
dc.description.abstractIn this study, we conducted an extensive investigation of the biodegradation capabilities and stress response of the newly isolated strain Pseudomonas veronii SM-20 in order, to assess its potential for bioremediation of sites contaminated with polycyclic aromatic hydrocarbons (PAHs). Initially, phenotype microarray technology demonstrated the strain's proficiency in utilizing various carbon sources and its resistance to certain stressors. Genomic analysis has identified numerous genes involved in aromatic hydrocarbon metabolism. Biodegradation assay analyzed the depletion of phenanthrene (PHE) when it was added as a sole carbon and energy source. We found that P. veronii strain SM-20 degraded approximately 25% of PHE over a 30-day period, starting with an initial concentration of 600 mu g/mL, while being utilized for growth. The degradation process involved PHE oxidation to an unstable arene oxide and 9,10-phenanthrenequinone, followed by ring-cleavage. Comparative proteomics provided a comprehensive understanding of how the entire proteome responded to PHE exposure, revealing the strain's adaptation in terms of aromatic metabolism, surface properties, and defense mechanism. In conclusion, our findings shed light on the promising attributes of P. veronii SM-20 and offer valuable insights for the use of P. veronii species in environmental restoration efforts targeting PAH-impacted sites.en
dc.language.isoen
dc.relation.urlhttps://doi.org/10.3390/microorganisms12040753
dc.rightsCreative Commons Uveďte původ 4.0 Internationalcs
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleProteogenomic Characterization of Pseudomonas veronii SM-20 Growing on Phenanthrene as Only Carbon and Energy Sourceen
dcterms.accessRightsopenAccess
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/legalcode
dc.date.updated2024-10-07T08:46:07Z
dc.subject.keywordPseudomonasen
dc.subject.keywordbiodegradationen
dc.subject.keywordpolycyclic aromatic hydrocarbons (PAHs)en
dc.subject.keywordphenanthrene (PHE)en
dc.subject.keywordproteomicsen
dc.subject.keywordgenomicsen
dc.identifier.eissn2076-2607
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/UK/COOP/COOP
dc.date.embargoStartDate2024-10-07
dc.type.obd73
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.3390/microorganisms12040753
dc.identifier.utWos001211416300001
dc.identifier.eidScopus2-s2.0-85191438717
dc.identifier.obd648543
dc.identifier.pubmed38674697
dc.subject.rivPrimary10000::10600::10606
dcterms.isPartOf.nameMicroorganisms
dcterms.isPartOf.issn2076-2607
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume12
dcterms.isPartOf.journalIssue4
uk.faculty.primaryId115
uk.faculty.primaryNamePřírodovědecká fakultacs
uk.faculty.primaryNameFaculty of Scienceen
uk.department.primaryId1034
uk.department.primaryNameKatedra genetiky a mikrobiologiecs
uk.department.primaryNameDepartment of Genetics and Microbiologyen
dc.type.obdHierarchyCsČLÁNEK V ČASOPISU::článek v časopisu::původní článekcs
dc.type.obdHierarchyEnJOURNAL ARTICLE::journal article::original articleen
dc.type.obdHierarchyCode73::152::206en
uk.displayTitleProteogenomic Characterization of <em>Pseudomonas veronii</em> SM-20 Growing on Phenanthrene as Only Carbon and Energy Sourceen


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