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Relationship between yeast virus like elements and poxviruses at the transcriptional level

dc.contributor.authorVopálenský, Václav
dc.contributor.authorSýkora, Michal
dc.contributor.authorMělková, Zora
dc.contributor.authorBarvík, Ivan
dc.contributor.authorMašek, Tomáš
dc.contributor.authorHoráčková, Kamila
dc.contributor.authorPospíšek, Martin
dc.date.accessioned2024-10-14T09:15:47Z
dc.date.available2024-10-14T09:15:47Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2656
dc.description.abstractYeast cytoplasmic double-stranded DNA virus-like elements (VLEs, also known as linear plasmids) were found in a number of yeast species belonging to nine genera. The Kluyveromyces lactis pGKL1/2 plasmids, which serve as archetypes of yeast linear plasmids, are peculiar in many respects. Both plasmids are cytoplasmically localized, possess proteins covalently linked to their terminal inverted repeats and their compact genomes code for 15 genes in total including a killer toxin, two DNA polymerases, an RNA polymerase, a DNA helicase and a capping enzyme.Functions of most of the genes are putative and have not been assigned to them experimentally yet. We and others have found, that the RNA polymerase encoded by pGKL plasmids, as well as the capping enzyme and RNA helicase, are similar to the corresponding enzyme encoded by vaccinia virus (VACV). We have recently described unique structures of yeast VLEs mRNAs, we found that although these VLEs encode their own putative capping enzyme, only a few VLEs genes code for 5'-capped transcripts and that all of the VLE specific mRNAs are not 3'-polyadenylated. Surprisingly, the majority of VLE promoters give rise to uncapped mRNAs starting with short poly(A) sequences at their 5' ends that are not complementary to the plasmid DNA.en
dc.language.isoen
dc.rightsCreative Commons Uveďte původ 4.0 Internationalcs
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleRelationship between yeast virus like elements and poxviruses at the transcriptional levelen
dcterms.accessRightsopenAccess
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/legalcode
dc.date.updated2024-10-14T09:15:47Z
dc.subject.keywordYeasten
dc.subject.keywordRNAen
dc.subject.keywordVLEsen
dc.subject.keywordvirusen
dc.identifier.eissn2336-7210
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//LX22NPO5103
dc.date.embargoStartDate2024-10-14
dc.type.obd110
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.obd653773
dc.subject.rivPrimary10000::10600::10607
dcterms.isPartOf.nameCzech Chemical Society Symposium Series
dcterms.isPartOf.issn2336-7202
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume22
dcterms.isPartOf.journalIssue6
uk.faculty.primaryId115
uk.faculty.primaryNamePřírodovědecká fakultacs
uk.faculty.primaryNameFaculty of Scienceen
uk.faculty.secondaryId108
uk.faculty.secondaryName1. lékařská fakultacs
uk.faculty.secondaryNameFirst Faculty of Medicineen
uk.department.primaryId1034
uk.department.primaryNameKatedra genetiky a mikrobiologiecs
uk.department.primaryNameDepartment of Genetics and Microbiologyen
uk.department.secondaryId1539
uk.department.secondaryNameÚstav imunologie a mikrobiologie 1. LF UK a VFNcs
uk.department.secondaryNameInstitute of Immunology and Microbiologyen
uk.event.nameThe third annual meeting of the National Institute of Virology and Bacteriology (NIVB)
dc.description.pageRange243-243
dc.type.obdHierarchyCsABSTRAKT::abstrakt::abstrakt v elektronické podoběcs
dc.type.obdHierarchyEnABSTRACT::abstract::abstract in e-formen
dc.type.obdHierarchyCode110::130::136en
uk.displayTitleRelationship between yeast virus like elements and poxviruses at the transcriptional levelen


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