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Unveiling viral diversity through wholevirome sequencing

dc.contributor.authorSaláková, Martina
dc.contributor.authorKadlečková, Dominika
dc.contributor.authorŠimić, Ivana
dc.contributor.authorTachezy, Ruth
dc.date.accessioned2024-10-14T09:45:50Z
dc.date.available2024-10-14T09:45:50Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2660
dc.description.abstractIn the dynamic landscape of viral metagenomics, our research focuses on whole-virome sequencing using the Novel enrichment technique of viromes protocol (NetoVir protocol, 1), which we have adapted to diverse starting materials. The NetoVir protocol is a robust and fast approach for efficiently identifying DNA and RNA viruses without structural or genome preferences, ranging from small Picornaviridae to large viruses of the Mimiviridae family. The individual steps of the protocol allow for the enrichment of capsid-protected viruses, and through random nucleic acid amplification, sufficient reads can be obtained for subsequent bioinformatic analysis. Using a comprehensive bioinformatics, we can obtain information on a wide range of viruses without limiting our investigation to specific virus groups. This allows us to gain insight not only into known but also into unknown viruses.en
dc.language.isoen
dc.publisherČeská společnost chemická
dc.rightsCreative Commons Uveďte původ 4.0 Internationalcs
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleUnveiling viral diversity through wholevirome sequencingen
dcterms.accessRightsopenAccess
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/legalcode
dc.date.updated2024-10-14T09:45:50Z
dc.subject.keywordviralen
dc.subject.keywordDNAen
dc.identifier.eissn2336-7210
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//LX22NPO5103
dc.date.embargoStartDate2024-10-14
dc.type.obd110
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.obd653781
dc.subject.rivPrimary10000::10600::10607
dcterms.isPartOf.nameCzech Chemical Society Symposium Series
dcterms.isPartOf.issn2336-7202
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume22
dcterms.isPartOf.journalIssue6
uk.faculty.primaryId115
uk.faculty.primaryNamePřírodovědecká fakultacs
uk.faculty.primaryNameFaculty of Scienceen
uk.department.primaryId1034
uk.department.primaryNameKatedra genetiky a mikrobiologiecs
uk.department.primaryNameDepartment of Genetics and Microbiologyen
uk.event.nameThe third annual meeting of the National Institute of Virology and Bacteriology (NIVB)
dc.description.pageRange244-244
dc.type.obdHierarchyCsABSTRAKT::abstrakt::abstrakt v elektronické podoběcs
dc.type.obdHierarchyEnABSTRACT::abstract::abstract in e-formen
dc.type.obdHierarchyCode110::130::136en
uk.displayTitleUnveiling viral diversity through wholevirome sequencingen


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