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Developmental expression of catecholamine system in the human placenta and rat fetoplacental unit

dc.contributor.authorKarahoda, Rona
dc.contributor.authorVáchalová, Veronika
dc.contributor.authorPortillo, Ramón
dc.contributor.authorMahrla, Filip
dc.contributor.authorViňas-Noguera, Mireia
dc.contributor.authorAbad, Cilia
dc.contributor.authorŠtaud, František
dc.date.accessioned2024-12-02T14:10:55Z
dc.date.available2024-12-02T14:10:55Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2729
dc.description.abstractCatecholamines norepinephrine and dopamine have been implicated in numerous physiological processes within the central nervous system. Emerging evidence has highlighted the importance of tightly regulated monoamine levels for placental functions and fetal development. However, the complexities of synthesis, release, and regulation of catecholamines in the fetoplacental unit have not been fully unraveled. In this study, we investigated the expression of enzymes and transporters involved in synthesis, degradation, and transport of norepinephrine and dopamine in the human placenta and rat fetoplacental unit. Quantitative PCR and Western blot analyses were performed in early-to-late gestation in humans (first trimester vs. term placenta) and mid-to-late gestation in rats (placenta and fetal brain, intestines, liver, lungs, and heart). In addition, we analyzed the gene expression patterns in isolated primary trophoblast cells from the human placenta and placenta-derived cell lines (HRP-1, BeWo, JEG-3). In both human and rat placentas, the study identifies the presence of only PNMT, COMT, and NET at the mRNA and protein levels, with the expression of PNMT and NET showing gestational age dependency. On the other hand, rat fetal tissues consistently express the catecholamine pathway genes, revealing distinct developmental expression patterns. Lastly, we report significant transcriptional profile variations in different placental cell models, emphasizing the importance of careful model selection for catecholamine metabolism/transport studies. Collectively, integrating findings from humans and rats enhances our understanding of the dynamic regulatory mechanisms that underlie catecholamine dynamics during pregnancy. We identified similar patterns in both species across gestation, suggesting conserved molecular mechanisms and potentially shedding light on shared biological processes influencing placental development.en
dc.language.isoen
dc.relation.urlhttp://www.nature.com/articles/s41598-024-57481-5
dc.rightsCreative Commons Uveďte původ 4.0 Internationalcs
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleDevelopmental expression of catecholamine system in the human placenta and rat fetoplacental uniten
dcterms.accessRightsopenAccess
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/legalcode
dc.date.updated2024-12-13T17:40:45Z
dc.subject.keywordPlacentaen
dc.subject.keywordMonoaminesen
dc.subject.keywordTrophoblasten
dc.subject.keywordMetabolismen
dc.subject.keywordIn vitro modelsen
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//LX22NPO5107
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//SVV260663
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/UK/COOP/COOP
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/GA0/GA/GA20-13017S
dc.date.embargoStartDate2024-12-13
dc.type.obd73
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/s41598-024-57481-5
dc.identifier.utWos001190086900011
dc.identifier.eidScopus2-s2.0-85188441838
dc.identifier.obd655759
dc.identifier.pubmed38521816
dc.subject.rivPrimary30000::30100::30104
dcterms.isPartOf.nameScientific Reports
dcterms.isPartOf.issn2045-2322
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume14
dcterms.isPartOf.journalIssue1
uk.faculty.primaryId113
uk.faculty.primaryNameFarmaceutická fakulta v Hradci Královécs
uk.faculty.primaryNameFaculty of Pharmacy in Hradec Kraloveen
uk.department.primaryId371
uk.department.primaryNameKatedra farmakologie a toxikologiecs
uk.department.primaryNameDeparment of Pharmacology and Toxicologyen
uk.department.secondaryId113
uk.department.secondaryNameFarmaceutická fakulta v Hradci Královécs
uk.department.secondaryNameFaculty of Pharmacy in Hradec Kraloveen
dc.type.obdHierarchyCsČLÁNEK V ČASOPISU::článek v časopisu::původní článekcs
dc.type.obdHierarchyEnJOURNAL ARTICLE::journal article::original articleen
dc.type.obdHierarchyCode73::152::206en
uk.displayTitleDevelopmental expression of catecholamine system in the human placenta and rat fetoplacental uniten


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