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Antibiotic-resistant Escherichia coli from treated municipal wastewaters and Black-headed Gull nestlings on the recipient river

dc.contributor.authorMasarikova, Martina
dc.contributor.authorSukkar, Iva
dc.contributor.authorJamborova, Ivana
dc.contributor.authorMedvecky, Matej
dc.contributor.authorPapousek, Ivo
dc.contributor.authorLiterak, Ivan
dc.contributor.authorCizek, Alois
dc.contributor.authorDolejská, Monika
dc.date.accessioned2025-01-07T08:11:02Z
dc.date.available2025-01-07T08:11:02Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2778
dc.description.abstractWastewaters belong among the most important sources of environmental pollution, including antibiotic-resistant bacteria. The aim of the study was to evaluate treated wastewaters as a possible transmission pathway for bacterial colonisation of gulls occupying the receiving river. A collection of antibiotic-resistant Escherichia coli originating both from treated municipal wastewaters discharged to the river Svratka (Czech Republic) and nestlings of Black-headed Gull (Chroicocephalus ridibundus) living 35 km downstream of the outlet was obtained using selective cultivation. Isolates were further characterised by various phenotyping and genotyping methods. From a total of 670 E. coli isolates (450 from effluents, 220 from gulls), 86 isolates (41 from effluents, 45 from gulls) showed identical antibiotic resistance phenotype and genotype and were further analysed for clonal relatedness using pulsed-field gel electrophoresis (PFGE). Despite the overall high diversity of the isolates, 21 isolates from both sources showed similar PFGE profiles. Isolates belonging to epidemiologically important sequence types (ST131, 15 isolates; ST23, three isolates) were subjected to whole-genome sequencing. Subsequent phylogenetic analysis did not reveal any close clonal relationship between the isolates from the effluents and gulls' nestlings with the closest strains showing 90 SNPs difference. Although our study did not provide direct evidence of transmission of antibiotic-resistant E. coli to wild gulls via treated wastewaters, we observed gull chicks as carriers of diverse multi-resistant E. coli, including high-risk clones, posing risk of further bacterial contamination of the surrounding environment.en
dc.language.isoen
dc.relation.urlhttps://doi.org/10.1016/j.onehlt.2024.100901
dc.rightsCreative Commons Uveďte původ 4.0 Internationalcs
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.titleAntibiotic-resistant Escherichia coli from treated municipal wastewaters and Black-headed Gull nestlings on the recipient riveren
dcterms.accessRightsembargoedAccess
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/legalcode
dc.date.updated2025-01-07T08:11:02Z
dc.subject.keywordEnterobacteralesen
dc.subject.keywordEnvironmenten
dc.subject.keywordWhole-genome sequencingen
dc.subject.keywordWild birdsen
dc.identifier.eissn2352-7714
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/UK/COOP/COOP
dc.date.embargoStartDate2025-01-07
dc.date.embargoEndDate2024-12-17
dc.type.obd73
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.doi10.1016/j.onehlt.2024.100901
dc.identifier.utWos001325021800001
dc.identifier.eidScopus2-s2.0-85204725532
dc.identifier.obd653328
dc.subject.rivPrimary30000::30300::30303
dcterms.isPartOf.nameOne Health
dcterms.isPartOf.issn2352-7714
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume19
dcterms.isPartOf.journalIssueDecember
uk.faculty.primaryId111
uk.faculty.primaryNameLékařská fakulta v Plznics
uk.faculty.primaryNameFaculty of Medicine in Pilsenen
uk.department.primaryId100012968318
uk.department.primaryNameBiomedicínské centrumcs
uk.department.primaryNameBiomedical Centeren
dc.type.obdHierarchyCsČLÁNEK V ČASOPISU::článek v časopisu::původní článekcs
dc.type.obdHierarchyEnJOURNAL ARTICLE::journal article::original articleen
dc.type.obdHierarchyCode73::152::206en
uk.displayTitleAntibiotic-resistant Escherichia coli from treated municipal wastewaters and Black-headed Gull nestlings on the recipient riveren


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