UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine
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Author
Vrablikova, Alena
Fojtikova, Martina
Hezova, Renata
Simeckova, Pavlina
Brezani, Veronika
Strakova, Nicol
Kotoucek, Jan
Masek, Josef
Psikal, Ivan
Publication date
2023Published in
Scientific ReportsVolume / Issue
13 (1)ISBN / ISSN
ISSN: 2045-2322Metadata
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This publication has a published version with DOI 10.1038/s41598-023-46791-9
Abstract
Ultraviolet irradiation is an effective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fluorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m2 was determined. At a dose of 36.8 J/m2, only 0.5% of GFP-expressing cells were detected by flow cytometry and confocal microscopy. The stability of purified VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m2. Up to the dose of 11 J/m2, no significant effect of UV-C light on the stability of VP1-PCV2bCap was detected. We observed a dose-dependent increase in VP1-PCV2bCap-specific immune response in BALB/c mice immunized by recombinant protein sterilized by irradiation in dose 11 J/m2 with no significant difference between vaccines sterilized by UV-C light and filtration. A substantial difference in the production of VP1-PCV2bCap specific IgG was observed in piglets immunized with VP1-PCV2bCap sterilized by UV-C in comparison with protein sterilized by filtration in combination with the inactivation of baculovirus by binary ethylenimine. UV-C irradiation represents an effective method for vaccine sterilization, where commonly used methods of sterilization are not possible.
Keywords
UV-C, irradiation, VP1-PCV2bCap, vaccine, virus
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https://hdl.handle.net/20.500.14178/2261License
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