A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Autor
Vozniuk, Oleksandra
Datum vydání
2024Publikováno v
ChemistryOpenRočník / Číslo vydání
13 (3)ISBN / ISSN
ISSN: 2191-1363ISBN / ISSN
eISSN: 2191-1363Informace o financování
MSM//LX22NPO5107
UK/UNCE/MED/UNCE/MED/007
UK/PROGRES/Q26
UK/PROGRES/Q27
MSM/LM/LM2023053
TA0/TN/TN02000109
MZ0/NU/NU21-08-00407
MZ0/NU/NU22-D-136
MZ0/NU/NU22-08-00160
TA0/TN/TN02000109
MZ0/NU/NU21-08-00407
Metadata
Zobrazit celý záznamKolekce
Tato publikace má vydavatelskou verzi s DOI 10.1002/open.202300147
Abstrakt
A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 mu g/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro. A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media. Separation was performed using a gradient on a C18 column with a mobile phase of acetonitrile and acidified miliQ water. The method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, as well as for robustness. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.image
Klíčová slova
HPLC/UV, ketoprofen, cellular medium,
Trvalý odkaz
https://hdl.handle.net/20.500.14178/2636Licence
Licence pro užití plného textu výsledku: Creative Commons Uveďte původ 4.0 International