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Dear users, welcome to the new interface of the Charles University Publication Repository. We would appreciate it if you could take a moment of your time to provide us with feedback on the new design of the repository.
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Improvement of the signal to noise ratio for fluorescent imaging in microfluidic chips
dc.contributor.author | Liu, Xiaocheng | |
dc.contributor.author | Zhu, Hanliang | |
dc.contributor.author | Sabó, Ján | |
dc.contributor.author | Lánský, Zdeněk | |
dc.contributor.author | Neužil, Pavel | |
dc.date.accessioned | 2023-03-28T09:10:31Z | |
dc.date.available | 2023-03-28T09:10:31Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1822 | |
dc.description.abstract | Microfluidics systems can be fabricated in various ways using original silicon glass systems, with easy Si processing and surface modifications for subsequent applications such as cell seeding and their study. Fluorescent imaging of cells became a standard technique for the investigation of cell behavior. Unfortunately, high sensitivity fluorescent imaging, e.g., using total internal reflection fluorescence (TIRF) microscopy, is problematic in these microfluidic systems because the uneven surfaces of the silicon channels' bottoms affect light penetration through the optical filters. In this work, we study the nature of the phenomenon, finding that the problem can be rectified by using a silicon-on-insulator (SOI) substrate, defining the channel depth by the thickness of the top Si layer, and halting the etching at the buried SiO2 layer. Then the fluorescent background signal drops by = 5 times, corresponding to the limit of detection drop from = 0.05 mM to = 50 nM of fluorescein. We demonstrate the importance of a flat surface using TIRF-based single-molecule detection, improving the signal to a noise ratio more than 18 times compared to a conventional Si wafer. Overall, using very high-quality SOI substrates pays off, as it improves the fluorescence image quality due to the increase in signal-to-noise ratio. Concerning the cost of microfluidic device fabrication-design, mask fabrication, wafer processing, and device testing-the initial SOI wafer cost is marginal, and using it improves the system performance. | en |
dc.language.iso | en | |
dc.relation.url | https://doi.org/10.1038/s41598-022-23426-z | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Improvement of the signal to noise ratio for fluorescent imaging in microfluidic chips | en |
dcterms.accessRights | openAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2023-10-02T06:15:44Z | |
dc.subject.keyword | electrochemical impedance spectroscopy | en |
dc.subject.keyword | X-ray-fluorescence | en |
dc.subject.keyword | surface-roughness | en |
dc.subject.keyword | electrochemiluminescence | en |
dc.subject.keyword | proteins | en |
dc.subject.keyword | progress | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/PROGRES/Q46 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/SVV/SVV260567 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/GAUK/GAUK276321 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM/LM/LM2018129 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM/EF/EF18_046/0016045 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/COOP/COOP | |
dc.date.embargoStartDate | 2023-10-02 | |
dc.type.obd | 73 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.doi | 10.1038/s41598-022-23426-z | |
dc.identifier.utWos | 000879914800037 | |
dc.identifier.eidScopus | 2-s2.0-85141545710 | |
dc.identifier.obd | 623600 | |
dc.identifier.riv | RIV/00216208:11310/22:10455940 | |
dc.identifier.pubmed | 36344576 | |
dc.subject.rivPrimary | 10000::10400::10403 | |
dcterms.isPartOf.name | Scientific Reports | |
dcterms.isPartOf.issn | 2045-2322 | |
dcterms.isPartOf.journalYear | 2022 | |
dcterms.isPartOf.journalVolume | 12 | |
dcterms.isPartOf.journalIssue | 1 | |
uk.faculty.primaryId | 115 | |
uk.faculty.primaryName | Přírodovědecká fakulta | cs |
uk.faculty.primaryName | Faculty of Science | en |
uk.department.primaryId | 1049 | |
uk.department.primaryName | Katedra fyzikální a makromolekulární chemie | cs |
uk.department.primaryName | Department of Physical and Macromolecular Chemistry | en |
uk.department.secondaryId | 1035 | |
uk.department.secondaryName | Katedra buněčné biologie | cs |
uk.department.secondaryName | Department of Cell Biology | en |
dc.type.obdHierarchyCs | ČLÁNEK V ČASOPISU::článek v časopisu::původní článek | cs |
dc.type.obdHierarchyEn | JOURNAL ARTICLE::journal article::original article | en |
dc.type.obdHierarchyCode | 73::152::206 | en |
uk.displayTitle | Improvement of the signal to noise ratio for fluorescent imaging in microfluidic chips | en |