dc.contributor.author | Študentová, Vendula | |
dc.contributor.author | Sudová, Vendula | |
dc.contributor.author | Bitar, Ibrahim | |
dc.contributor.author | Pašková, Veronika | |
dc.contributor.author | Moravec, Jiří | |
dc.contributor.author | Pompach, Petr | |
dc.contributor.author | Volný, Michael | |
dc.contributor.author | Novák, Petr | |
dc.contributor.author | Hrabák, Jaroslav | |
dc.contributor.editor | Šímová, Šárka | |
dc.date.accessioned | 2023-06-29T06:40:20Z | |
dc.date.available | 2023-06-29T06:40:20Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1960 | |
dc.description.abstract | The resistance to carbapenems is usually mediated by enzymes hydrolyzing β-lactam ring. Recently, an alternative way of the modification of the antibiotic, a β-lactone formation by OXA-48-like enzymes, in some carbapenems was identified. We focused our study on a deep analysis of OXA-48-like-producing Enterobacterales, especially strains showing poor hydrolytic activity. In this study, well characterized 74 isolates of Enterobacterales resistant to carbapenems were used. Carbapenemase activity was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), liquid chromatography/mass spectrometry (LC-MS), Carba-NP test and modified Carbapenem Inactivation Method (mCIM). As meropenem-derived β-lactone possesses the same molecular weight as native meropenem (MW 383.46 g/mol), β-lactonization cannot be directly detected by MALDI-TOF MS. In the spectra, however, the peaks of m/z = 340.5 and 362.5 representing decarboxylated β-lactone and its sodium adduct were detected in 25 out of 35 OXA-48-like producers. In the rest 10 isolates, decarboxylated hydrolytic product (m/z = 358.5) and its sodium adduct (m/z = 380.5) have been detected. The peak of m/z = 362.5 was detected in 3 strains co-producing OXA-48-like and NDM-1 carbapenemases. The respective signal was identified in no strain producing class A or class B carbapenemase alone showing its specificity for OXA-48-like carbapenemases. Using LC-MS, we were able to identify meropenem-derived β-lactone directly according to the different retention time. All strains with a predominant β-lactone production showed negative results of Carba NP test. In this study, we have demonstrated that the strains producing OXA-48-like carbapenemases showing false-negative results using Carba NP test and MALDI-TOF MS preferentially produced meropenem-derived β-lactone. We also identified β-lactone-specific peak in MALDI-TOF MS spectra and demonstrated the ability of LC-MS to detect meropenem-derived β-lactone. | en |
dc.language.iso | en | |
dc.relation.url | http://www.ccsss.cz/index.php/ccsss/issue/view/37/67 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Preferred β-lactone synthesis can explain high rate of false-negative results in the detection of OXA-48-like carbapenemases | en |
dcterms.accessRights | embargoedAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2023-10-02T06:17:29Z | |
dc.subject.keyword | β-lactone | en |
dc.subject.keyword | OXA-48-like | en |
dc.subject.keyword | carbapenemase | en |
dc.subject.keyword | MALDI-TOF | en |
dc.subject.keyword | OXA-48 | en |
dc.subject.keyword | beta-lactamase | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM//LX22NPO5103 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MZ0/NV/NV19-05-00541 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM/EF/EF16_019/0000787 | |
dc.date.embargoStartDate | 2023-10-02 | |
dc.date.embargoEndDate | 2023-06-28 | |
dc.type.obd | 110 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.obd | 632843 | |
dc.subject.rivPrimary | 30000::30300::30303 | |
dcterms.isPartOf.name | Czech Chemical Society Symposium Series | |
dcterms.isPartOf.issn | 2336-7202 | |
dcterms.isPartOf.journalYear | 2022 | |
dcterms.isPartOf.journalVolume | 20 | |
dcterms.isPartOf.journalIssue | 6 | |
uk.faculty.primaryId | 111 | |
uk.faculty.primaryName | Lékařská fakulta v Plzni | cs |
uk.faculty.primaryName | Faculty of Medicine in Pilsen | en |
uk.department.primaryId | 100012968318 | |
uk.department.primaryName | Biomedicínské centrum | cs |
uk.department.primaryName | Biomedical Center | en |
uk.department.secondaryId | 1356 | |
uk.department.secondaryId | 1359 | |
uk.department.secondaryName | Ústav klinické biochemie a hematologie | cs |
uk.department.secondaryName | Department of Clinical Biochemistry and Haematology | en |
uk.department.secondaryName | Ústav mikrobiologie | cs |
uk.department.secondaryName | Department of Microbiology | en |
uk.event.name | The first annual meeting of the National Institute of Virology and Bacteriology | |
dc.description.pageRange | 395-395 | |
dc.type.obdHierarchyCs | ABSTRAKT::abstrakt::abstrakt v konferenčním sborníku | cs |
dc.type.obdHierarchyEn | ABSTRACT::abstract::abstract in conference proceedings | en |
dc.type.obdHierarchyCode | 110::130::462 | en |
uk.displayTitle | Preferred β-lactone synthesis can explain high rate of false-negative results in the detection of OXA-48-like carbapenemases | en |