dc.contributor.author | Aloba, Bisola K | |
dc.contributor.author | Kinnevey, Peter M | |
dc.contributor.author | Monecke, Stefan | |
dc.contributor.author | Brennan, Gráinne I | |
dc.contributor.author | O'Connell, Brian | |
dc.contributor.author | Blomfeldt, Anita | |
dc.contributor.author | McManus, Brenda A | |
dc.contributor.author | Schneider-Brachert, Wulf | |
dc.contributor.author | Tkadlec, Jan | |
dc.contributor.author | Ehricht, Ralf | |
dc.contributor.author | Senok, Abiola | |
dc.contributor.author | Bartels, Mette Damkjær | |
dc.contributor.author | Coleman, David C | |
dc.date.accessioned | 2023-07-26T10:10:25Z | |
dc.date.available | 2023-07-26T10:10:25Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1985 | |
dc.description.abstract | BACKGROUND: A novel Panton-Valentine leukocidin (PVL)-positive meticillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC)5-MRSA-IVc ('Sri Lankan' clone) was recently described from Sri Lanka. Similar isolates caused a recent Irish hospital outbreak. AIMS: To investigate the international dissemination and diversity of PVL-positive CC5-MRSA-IVc isolates from hospital and community settings using whole-genome sequencing (WGS). METHODS: Core-genome single nucleotide polymorphic (cgSNP) analysis and multilocus sequence typing (cgMLST) and microarray-based detection of antimicrobial-resistance and virulence genes were used to investigate PVL-positive CC5-MRSA-IVc (N=214 including 46 'Sri Lankan' clone) from hospital and community settings in 12 countries over 17 years. Comparators included 29 PVL-positive and 23 PVL-negative CC5/ST5-MRSA-I/II/IVa/IVc/IVg/V. FINDINGS: Maximum-likelihood cgSNP analysis grouped 209/214 (97.7%) CC5-MRSA-IVc into Clade I; average of 110 cgSNPs between isolates. Clade III contained the five remaining CC5-MRSA-IVc; average of 92 cgSNPs between isolates. Clade II contained seven PVL-positive CC5-MRSA-IVa comparators, whereas the remaining 45 comparators formed an outlier group. Minimum-spanning cgMLST analysis revealed a comparably low average of 57 allelic differences between all CC5/ST5-MRSA-IVc. All 214 CC5/ST5-MRSA-IVc were identified as 'Sri Lankan' clone, predominantly spa type t002 (186/214) with low population diversity and harboured a similar range of virulence genes and variable antimicrobial-resistance genes. All 214 Sri Lankan clone isolates and Clade II comparators harboured a 9,616 bp chromosomal PVL-encoding phage remnant, suggesting both arose from a PVL-positive meticillin-susceptible ancestor. Over half of Sri Lankan clone isolates were from infections (142/214), and where detailed metadata were available (168/214), most were community-associated (85/168). CONCLUSIONS: Stable chromosomal retention of pvl may facilitate Sri-Lankan clone dissemination. | en |
dc.language.iso | en | |
dc.relation.url | https://doi.org/10.1016/j.jhin.2022.11.015 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | An emerging Panton-Valentine leukocidin (PVL)-positive CC5-meticillin-resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing | en |
dcterms.accessRights | openAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2023-10-02T06:14:38Z | |
dc.subject.keyword | CC5-MRSA-IVc | en |
dc.subject.keyword | PVL | en |
dc.subject.keyword | Sri Lankan clone | en |
dc.subject.keyword | dissemination | en |
dc.subject.keyword | epidemiology | en |
dc.subject.keyword | phylogenomics | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM//LX22NPO5103 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/COOP/COOP | |
dc.date.embargoStartDate | 2023-10-02 | |
dc.type.obd | 73 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.doi | 10.1016/j.jhin.2022.11.015 | |
dc.identifier.utWos | 000920758900001 | |
dc.identifier.eidScopus | 2-s2.0-85145710218 | |
dc.identifier.obd | 619034 | |
dc.identifier.pubmed | 36481685 | |
dc.subject.rivPrimary | 30000::30300::30303 | |
dcterms.isPartOf.name | Journal of Hospital Infection | |
dcterms.isPartOf.issn | 0195-6701 | |
dcterms.isPartOf.journalYear | 2023 | |
dcterms.isPartOf.journalVolume | 132 | |
dcterms.isPartOf.journalIssue | February | |
uk.faculty.primaryId | 109 | |
uk.faculty.primaryName | 2. lékařská fakulta | cs |
uk.faculty.primaryName | Second Faculty of Medicine | en |
uk.department.primaryId | 109 | |
uk.department.primaryName | 2. lékařská fakulta | cs |
uk.department.primaryName | Second Faculty of Medicine | en |
uk.department.secondaryId | 1701 | |
uk.department.secondaryName | Ústav lékařské mikrobiologie | cs |
uk.department.secondaryName | Ústav lékařské mikrobiologie | en |
dc.description.pageRange | 8-19 | |
dc.type.obdHierarchyCs | ČLÁNEK V ČASOPISU::článek v časopisu::původní článek | cs |
dc.type.obdHierarchyEn | JOURNAL ARTICLE::journal article::original article | en |
dc.type.obdHierarchyCode | 73::152::206 | en |
uk.displayTitle | An emerging Panton-Valentine leukocidin (PVL)-positive CC5-meticillin-resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing | en |