dc.contributor.author | Iyyapan, Rajan | |
dc.contributor.author | Aleshkina, Daria | |
dc.contributor.author | Ming, Hao | |
dc.contributor.author | Dvořan, Michal | |
dc.contributor.author | Kakavand, Kianoush | |
dc.contributor.author | Jansová, Denisa | |
dc.contributor.author | del Llano Solanas, Edgar | |
dc.contributor.author | Gahurová, Lenka | |
dc.contributor.author | Bruce, Alexander William | |
dc.contributor.author | Mašek, Tomáš | |
dc.contributor.author | Pospíšek, Martin | |
dc.contributor.author | Horvat, Filip | |
dc.contributor.author | Kubelka, Michal | |
dc.contributor.author | Jiang, Zongliang | |
dc.contributor.author | Šušor, Andrej | |
dc.date.accessioned | 2024-01-29T17:10:41Z | |
dc.date.available | 2024-01-29T17:10:41Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/2224 | |
dc.description.abstract | Translation is critical for development as transcription in the oocyte and early embryo is silenced. To illustrate the translational changes during meiosis and consecutive two mitoses of the oocyte and early embryo, we performed a genome-wide translatome analysis. Acquired data showed significant and uniform activation of key translational initiation and elongation axes specific to M-phases. Although global protein synthesis decreases in M-phases, translation initiation and elongation activity increases in a uniformly fluctuating manner, leading to qualitative changes in translation regulation via the mTOR1/4F/eEF2 axis. Overall, we have uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to the translational regulation of specific mRNAs with different modes of polysomal occupancy/translation that are important for oocyte and embryo developmental competence. Our results provide new insights into the regulation of gene expression during oocyte meiosis as well as the first two embryonic mitoses and show how temporal translation can be optimized. This study is the first step towards a comprehensive analysis of the molecular mechanisms that not only control translation during early development, but also regulate translation-related networks employed in the oocyte-to-embryo transition and embryonic genome activation. | en |
dc.language.iso | en | |
dc.relation.url | https://doi.org/10.1093/nar/gkad996 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | The translational oscillation in oocyte and early embryo development | en |
dcterms.accessRights | openAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2024-02-18T20:40:38Z | |
dc.subject.keyword | translation | en |
dc.subject.keyword | early embryo | en |
dc.subject.keyword | mammalian oocyte | en |
dc.subject.keyword | messenger-RNA translation | en |
dc.subject.keyword | chromosome segregation | en |
dc.subject.keyword | S6 kinase | en |
dc.subject.keyword | phosphorylation | en |
dc.subject.keyword | maturation | en |
dc.subject.keyword | tolerance | en |
dc.subject.keyword | cancer | en |
dc.subject.keyword | eEF2 | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/COOP/COOP | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/GA0/GA/GA22-27301S | |
dc.date.embargoStartDate | 2024-02-18 | |
dc.type.obd | 73 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.doi | 10.1093/nar/gkad996 | |
dc.identifier.utWos | 001100405100001 | |
dc.identifier.eidScopus | 2-s2.0-85180007080 | |
dc.identifier.obd | 638868 | |
dc.identifier.pubmed | 37950888 | |
dc.subject.rivPrimary | 10000::10600 | |
dc.relation.datasetUrl | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE230016 | |
dcterms.isPartOf.name | Nucleic Acids Research | |
dcterms.isPartOf.issn | 0305-1048 | |
dcterms.isPartOf.journalYear | 2023 | |
dcterms.isPartOf.journalVolume | 51 | |
dcterms.isPartOf.journalIssue | 22 | |
uk.faculty.primaryId | 115 | |
uk.faculty.primaryName | Přírodovědecká fakulta | cs |
uk.faculty.primaryName | Faculty of Science | en |
uk.department.primaryId | 1034 | |
uk.department.primaryName | Katedra genetiky a mikrobiologie | cs |
uk.department.primaryName | Department of Genetics and Microbiology | en |
dc.description.pageRange | 12076-12091 | |
dc.type.obdHierarchyCs | ČLÁNEK V ČASOPISU::článek v časopisu::původní článek | cs |
dc.type.obdHierarchyEn | JOURNAL ARTICLE::journal article::original article | en |
dc.type.obdHierarchyCode | 73::152::206 | en |
uk.displayTitle | The translational oscillation in oocyte and early embryo development | en |