dc.contributor.author | Galati, Luisa | |
dc.contributor.author | Gupta, Purnima | |
dc.contributor.author | Tufaro, Antonio | |
dc.contributor.author | Marinaro, Mariarosaria | |
dc.contributor.author | Saponaro, Concetta | |
dc.contributor.author | Escobar Marcillo, David Israel | |
dc.contributor.author | Loisi, Donato | |
dc.contributor.author | Sen, Rajdip | |
dc.contributor.author | Robitaille, Alexis | |
dc.contributor.author | Brancaccio, Rosario N. | |
dc.contributor.author | Cuenin, Cyrille | |
dc.contributor.author | McKay-Chopin, Sandrine | |
dc.contributor.author | Paradiso, Angelo Virgilio | |
dc.contributor.author | Liška, Václav | |
dc.contributor.author | Souček, Pavel | |
dc.contributor.author | Zito, Francesco Alfredo | |
dc.contributor.author | Hughes, David J. | |
dc.contributor.author | Tommasino, Massimo | |
dc.contributor.author | Gheit, Tarik | |
dc.date.accessioned | 2024-01-31T14:40:43Z | |
dc.date.available | 2024-01-31T14:40:43Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/2227 | |
dc.description.abstract | Background: Although the role of viral agents, such as human papillomavirus (e.g. HPV16, HPV18) in colorectal cancer (CRC) has been previously investigated, results remain inconclusive. Methods: To further evaluate the involvement of oncogenic HPV types in CRC, 40 frozen neoplastic and 40 adjacent colonic tissues collected from Italian patients were analyzed by Luminex-based assays that detect a broad spectrum of HPV types, i.e. Alpha (n = 21), Beta (n = 46) and Gamma HPVs (n = 52). In addition, 125 frozen CRC samples and 70 surrounding mucosal tissues were collected from Czech patients and analyzed by broad spectrum PCR protocols: (i) FAP59/64, (ii) FAPM1 and (iii) CUT combined with Next Generation Sequencing (NGS). Results: Using Luminex-basedassays, DNA from HPV16 was detected in 5% (2/40) CRC tissues from Italian patients. One HPV16 DNA-positive CRC case was subsequently confirmed positive for E6*I mRNA. Cutaneous beta HPV types were detected in 10% (4/40) adjacent tissues only, namely HPV111 (n = 3) and HPV120 (n = 1), while gamma HPV168 (n = 1) and HPV199 (n = 1) types were detected in adjacent and in tumor tissues, respectively. The NGS analysis of the CRC Czech samples identified HPV sequences from mucosal alpha-3 (HPV89), alpha-7 (HPV18, 39, 68 and 70) and alpha-10 species (HPV11), as well as cutaneous beta-1 (HPV20, 24, 93, 98, 105,124) beta-2 (HPV23), beta-3 (HPV49) and gamma-1 species (HPV205). Conclusions: Our findings indicate that HPV types belonging to the mucosal alpha, and the 'cutaneous' beta and gamma genera can be detected in the colonic mucosal samples with a low prevalence rate and a low number of HPV reads by Luminex and NGS, respectively. However, additional studies are required to corroborate these findings. | en |
dc.language.iso | en | |
dc.relation.url | https://doi.org/10.1186/s13027-023-00552-5 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples | en |
dcterms.accessRights | openAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2024-01-31T19:40:47Z | |
dc.subject.keyword | Colorectal cancer | en |
dc.subject.keyword | HPV16 | en |
dc.subject.keyword | Human papillomavirus | en |
dc.subject.keyword | Luminex | en |
dc.subject.keyword | Next generation sequencing | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/COOP/COOP | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MZ0/NV/NV19-08-00113 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/UNCE/MED/UNCE/MED/006 | |
dc.date.embargoStartDate | 2024-01-31 | |
dc.type.obd | 73 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.doi | 10.1186/s13027-023-00552-5 | |
dc.identifier.utWos | 001098826200001 | |
dc.identifier.eidScopus | 2-s2.0-85175980032 | |
dc.identifier.obd | 638656 | |
dc.identifier.pubmed | 37941001 | |
dc.subject.rivPrimary | 30000::30200::30204 | |
dcterms.isPartOf.name | Infectious Agents and Cancer | |
dcterms.isPartOf.issn | 1750-9378 | |
dcterms.isPartOf.journalYear | 2023 | |
dcterms.isPartOf.journalVolume | 18 | |
dcterms.isPartOf.journalIssue | 1 | |
uk.faculty.primaryId | 111 | |
uk.faculty.primaryName | Lékařská fakulta v Plzni | cs |
uk.faculty.primaryName | Faculty of Medicine in Pilsen | en |
uk.faculty.secondaryId | 54 | |
uk.faculty.secondaryName | Fakultní nemocnice Plzeň | cs |
uk.faculty.secondaryName | University Hospital in Pilsen | en |
uk.department.primaryId | 100012968318 | |
uk.department.primaryName | Biomedicínské centrum | cs |
uk.department.primaryName | Biomedical Center | en |
uk.department.secondaryId | 1399 | |
uk.department.secondaryId | 5000002701 | |
uk.department.secondaryName | Chirurgická klinika | cs |
uk.department.secondaryName | Department of Surgery | en |
uk.department.secondaryName | Chirurgická klinika | cs |
uk.department.secondaryName | Department of Surgery | en |
dc.type.obdHierarchyCs | ČLÁNEK V ČASOPISU::článek v časopisu::původní článek | cs |
dc.type.obdHierarchyEn | JOURNAL ARTICLE::journal article::original article | en |
dc.type.obdHierarchyCode | 73::152::206 | en |
uk.displayTitle | Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples | en |