Virological characteristics of the SARS-CoV-2 Omicron EG.5.1 variant
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Autor
Tsujino, Shuhei
Deguchi, Sayaka
Nomai, Tomo
Padilla-Blanco, Miguel
Plianchaisuk, Arnon
Wang, Lei
Begum, Mst Monira
Uriu, Keiya
Mizuma, Keita
Nao, Naganori
Kojima, Isshu
Tsubo, Tomoya
Li, Jingshu
Matsumura, Yasufumi
Nagao, Miki
Oda, Yoshitaka
Tsuda, Masumi
Anraku, Yuki
Kita, Shunsuke
Yajima, Hisano
Sasaki-Tabata, Kaori
Guo, Ziyi
Hinay, Alfredo A Jr
Yoshimatsu, Kumiko
Yamamoto, Yuki
Nagamoto, Tetsuharu
Asakura, Hiroyuki
Nagashima, Mami
Sadamasu, Kenji
Yoshimura, Kazuhisa
Nasser, Hesham
Jonathan, Michael
Putri, Olivia
Kim, Yoonjin
Chen, Luo
Suzuki, Rigel
Tamura, Tomokazu
Maenaka, Katsumi
Irie, Takashi
Matsuno, Keita
Tanaka, Shinya
Ito, Jumpei
Ikeda, Terumasa
Takayama, Kazuo
Hashiguchi, Takao
Fukuhara, Takasuke
Sato, Kei
Datum vydání
2024Publikováno v
Microbiology and immunologyRočník / Číslo vydání
68 (9)ISBN / ISSN
ISSN: 0385-5600ISBN / ISSN
eISSN: 1348-0421Metadata
Zobrazit celý záznamKolekce
Tato publikace má vydavatelskou verzi s DOI 10.1111/1348-0421.13165
Abstrakt
In middle to late 2023, a sublineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron XBB, EG.5.1 (a progeny of XBB.1.9.2), is spreading rapidly around the world. We performed multiscale investigations, including phylogenetic analysis, epidemic dynamics modeling, infection experiments using pseudoviruses, clinical isolates, and recombinant viruses in cell cultures and experimental animals, and the use of human sera and antiviral compounds, to reveal the virological features of the newly emerging EG.5.1 variant. Our phylogenetic analysis and epidemic dynamics modeling suggested that two hallmark substitutions of EG.5.1, S:F456L and ORF9b:I5T are critical to its increased viral fitness. Experimental investigations on the growth kinetics, sensitivity to clinically available antivirals, fusogenicity, and pathogenicity of EG.5.1 suggested that the virological features of EG.5.1 are comparable to those of XBB.1.5. However, cryo-electron microscopy revealed structural differences between the spike proteins of EG.5.1 and XBB.1.5. We further assessed the impact of ORF9b:I5T on viral features, but it was almost negligible in our experimental setup. Our multiscale investigations provide knowledge for understanding the evolutionary traits of newly emerging pathogenic viruses, including EG.5.1, in the human population.
Klíčová slova
COVID‐19, EG.5.1, ORF9b, Omicron, SARS‐CoV‐2, pathogenicity
Trvalý odkaz
https://hdl.handle.net/20.500.14178/2552Licence
Licence pro užití plného textu výsledku: Creative Commons Uveďte původ 4.0 International