dc.contributor.author | Bitar, Ibrahim | |
dc.contributor.author | Papagiannitsis, Costas C. | |
dc.contributor.author | Kraftová, Lucie | |
dc.contributor.author | Mattioni Marchetti, Vittoria | |
dc.contributor.author | Petinaki, Efthymia | |
dc.contributor.author | Finianos, Marc | |
dc.contributor.author | Chudějová, Kateřina | |
dc.contributor.author | Žemličková, Helena | |
dc.contributor.author | Hrabák, Jaroslav | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1642 | |
dc.description.abstract | Background: VIM metallo-β-lactamases are enzymes characterized by the ability to hydrolyze all β-lactams. Usually, blaVIM-like genes are carried by class 1 integrons. In the Czech Republic, only sporadic cases of VIM-producing Enterobacterales have been reported in which those isolates carried the VIM-1 carbapenemase-encoding integron In110. However, during 2019-2020, an increased number was reported. Therefore, the aim of the current study was to characterize the genetic elements involved in the increased spread of blaVIM genes.Materials and methods: 32 VIM-producing Enterobacterales collected between 2019 and 2020 were subjected to: antimicrobial susceptibility testing, integron analysis, and short reads sequencing. Based on the results, 19 isolates were selected as representative and sequenced using Sequel I platform.Results: The 32 VIM-producing isolates exhibited variations in the MICs of carbapenems. Based on short-read data, 26 of the 32 sequenced isolates harbored the blaVIM-1 allele while six isolates carried the blaVIM-4 gene. The most prevalent was the In110 integron (n = 24) and two isolates carried the In4873 class 1 integron. The blaVIM-4 allele was identified in class 1 integrons In1174 (n = 3), In416 (n = 1), In2143 (n = 1) and In2150. Long reads sequencing revealed that the blaVIM was carried by: pKPC-CAV1193-like (n = 6), HI1 (pNDM-CIT; n = 4), HI2 (n = 3), FIB (pECLA; n = 2) and N (n = 1) incompatibility groups. Two blaVIM-carrying plasmids could not be typed by the database, while another one was integrated into the chromosome.Conclusion: We observed the spread of VIM-encoding integrons, mainly of In110, among Enterobacterales isolated from Czech hospitals, but also an increased number of novel elements underlining the ongoing evolution. | en |
dc.language.iso | en | |
dc.relation.url | https://doi.org/10.3389/fmicb.2022.993240 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals | en |
dcterms.accessRights | embargoedAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2023-10-02T06:14:59Z | |
dc.subject.keyword | Enterobacterales | en |
dc.subject.keyword | whole-genome sequencing | en |
dc.subject.keyword | blaVIM-1 | en |
dc.subject.keyword | blaVIM-4 | en |
dc.subject.keyword | In110 | en |
dc.subject.keyword | plasmids | en |
dc.subject.keyword | integrons | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MZ0/NU/NU20J-05-00033 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM//LX22NPO5103 | |
dc.relation.fundingReference | info:eu-repo/grantAgreement/UK/COOP/COOP | |
dc.date.embargoStartDate | 2023-10-02 | |
dc.date.embargoEndDate | 2023-01-04 | |
dc.type.obd | 73 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.doi | 10.3389/fmicb.2022.993240 | |
dc.identifier.utWos | 000913341300001 | |
dc.identifier.eidScopus | 2-s2.0-85146390672 | |
dc.identifier.obd | 620554 | |
dc.identifier.pubmed | 36687644 | |
dc.subject.rivPrimary | 30000::30300::30303 | |
dc.relation.datasetUrl | https://www.frontiersin.org/articles/10.3389/fmicb.2022.993240/full#supplementary-material | |
dcterms.isPartOf.name | Frontiers in Microbiology | |
dcterms.isPartOf.issn | 1664-302X | |
dcterms.isPartOf.journalYear | 2023 | |
dcterms.isPartOf.journalVolume | 13 | |
dcterms.isPartOf.journalIssue | January | |
uk.faculty.primaryId | 111 | |
uk.faculty.primaryName | Lékařská fakulta v Plzni | cs |
uk.faculty.primaryName | Faculty of Medicine in Pilsen | en |
uk.faculty.secondaryId | 110 | |
uk.faculty.secondaryName | 3. lékařská fakulta | cs |
uk.faculty.secondaryName | Third Faculty of Medicine | en |
uk.department.primaryId | 1359 | |
uk.department.primaryName | Ústav mikrobiologie | cs |
uk.department.primaryName | Department of Microbiology | en |
uk.department.secondaryId | 100012968318 | |
uk.department.secondaryId | 100033743623 | |
uk.department.secondaryName | Biomedicínské centrum | cs |
uk.department.secondaryName | Biomedical Center | en |
uk.department.secondaryName | Ústav mikrobiologie 3. LF UK, FNKV a SZÚ | cs |
uk.department.secondaryName | Department of Microbiology 3FM CU, UHKV and NIPH | en |
dc.type.obdHierarchyCs | ČLÁNEK V ČASOPISU::článek v časopisu::původní článek | cs |
dc.type.obdHierarchyEn | JOURNAL ARTICLE::journal article::original article | en |
dc.type.obdHierarchyCode | 73::152::206 | en |
uk.displayTitle | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals | en |