Stimuli-responsive polypeptide nanogels for trypsin inhibition
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Author
Šálek, Petr
Dvořáková, Jana
Publication date
2022Published in
Beilstein Journal of NanotechnologyVolume / Issue
13 (June)ISBN / ISSN
ISSN: 2190-4286Metadata
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This publication has a published version with DOI 10.3762/bjnano.13.45
Abstract
A new type of hydrophilic, biocompatible, and biodegradable polypeptide nanogel depots loaded with the natural serine protease inhibitor α(1)-antitrypsin (AAT) was applied for the inhibition of the inflammatory mediator trypsin. Two types of nanogels were prepared from linear synthetic polypeptides based on biocompatible and biodegradable poly[N(5)-(2-hydroxyethyl)-L-glutamine-ran-N(5)-propargyl-L-glutamine-ran-N(5)-(6-aminohexyl)-L-glutamine]-ran-N(5)-[2-(4-hydroxyphenypethyl)-L-glutamine] (PHEG-Tyr) or biocompatible N(α)-L-lysine-grafted α,β-poly[(2-propyne)-D,L-aspartamide-ran-(2-hydroxyethyl)-DL-aspartamide-ran-(2-(4-hydroxyphenyl)ethyl)-DL-aspartamide] (N(α)-Lys-NG). Both nanogels were prepared by HRP/H2O2-mediated crosslinking in inverse miniemulsions with pH and temperature-stimuli responsive behavior confirmed by dynamic light scattering and zeta potential measurements. The loading capacity of PHEG-Tyr and N(α)-Lys-NG nanogels and their release profiles were first optimized with bovine serum albumin. The nanogels were then used for loading and release of AAT. PHEG-Tyr and N(α)-Lys-NG nanogels showed different loading capacities for AAT with the maximum (20%) achieved with N(α)-Lys-NG nanogel. In both cases, the nanogel depots demonstrated a burst release of AAT during the first 6 h, which could be favorable for quick inhibition of trypsin. A consequent pilot in vitro inhibition study revealed that both PHEG-Tyr and N(α)-Lys-NG nanogels loaded with AAT successfully inhibited the enzymatic activity of trypsin. Furthermore, the inhibitory efficiency of the AAT-loaded nanogels was higher than that of only AAT. Interestingly, also non-loaded PHEG-Tyr and N(α)-Lys-NG nanogels were shown to effectively inhibit trypsin because they contain suitable amino acids in their structures that effectively block the active site of trypsin.
Keywords
α(1)-antitrypsin, inflammatory mediator, nanogel, polypeptide, trypsin
Permanent link
https://hdl.handle.net/20.500.14178/1817License
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