dc.contributor.author | Kraftová, Lucie | |
dc.contributor.author | Finianos, Marc | |
dc.contributor.author | Heringová, Vendula | |
dc.contributor.author | Chudějová, Kateřina | |
dc.contributor.author | Jakubů, Vladislav | |
dc.contributor.author | Žemličková, Helena | |
dc.contributor.author | Costas, Papagiannitsis | |
dc.contributor.author | Bitar, Ibrahim | |
dc.contributor.author | Hrabák, Jaroslav | |
dc.contributor.editor | Šímová, Šárka | |
dc.date.accessioned | 2023-06-29T10:40:21Z | |
dc.date.available | 2023-06-29T10:40:21Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1962 | |
dc.description.abstract | Carbapenems are currently last-resort antibiotics for the therapy of infections caused by multidrug-resistant Gram-negative bacteria. Therefore, the resistence to those drugs represents a significant threat of current medicine. Genes encoding for carbapenemases are mainly spread on mobile-genetic elements, especially plasmids. As demonstrated by several studies, they can be efficiently spread in bacterial populations. For surveillance purpose, it is crucial to under-stand evolution and spread of those resistence determinants on molecular-genetic level. In the Czech Republic, car-bapenemase-producing bacteria are monitored in a routine level by diagnostic clinical laboratories and confirmed at Na-tional Reference Laboratory for Antibiotics of National Insti-tute of Public Health and at Biomedical Center of Faculty of Medicine in Pilsen, Charles University. Whole-genome-sequencing-based molecular surveillance of those bacteria has been established since 2014. Among three main molecular groups of carbapenemases, KPC-type enzymes are spread globally, causing high-level of resistence to carbapenems. In this study we present the ongoing spread of the KPC-producing strains, which is evolving to an epidemic in Czech hospitals. During the period of 2018-2019, a total of 108 KPC-producing Enterobacterales were recovered from 20 hospi-tals. Analysis of long-read sequencing data revealed the pres-ence of several types of blaKPC-carrying plasmids; 19 out of 25 blaKPC-carrying plasmids could be assigned to R (n = 12), N (n = 5), C (n = 1) and P6 (n = 1) incompatibility (Inc) groups. Five of the remaining blaKPC-carrying plasmids were multireplicon, while one plasmid couldn't be typed. Addition-ally, phylogenetic analysis confirmed the spread of blaKPC-carrying plasmids among different clones of diverse Entero-bacterales species. Our findings demonstrated that the in-creased prevalence of KPC-producing isolates was due to plasmids spreading among different species. In some districts, the local dissemination of IncR and IncN plasmids was ob-served. Additionally, the ongoing evolution of blaKPC-carrying plasmids, through genetic rearrangements, favours the preser-vation and further dissemination of these mobile genetic ele-ments. Therefore, the situation should be monitored, and im-mediate infection control should be implemented in hospitals reporting KPC-producing strains. | en |
dc.language.iso | en | |
dc.relation.url | http://www.ccsss.cz/index.php/ccsss/issue/view/37 | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Epidemic spread of KPC-producing bacteria in the Czech Republic | en |
dcterms.accessRights | embargoedAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2023-10-02T06:11:50Z | |
dc.subject.keyword | Carbapenemase | en |
dc.subject.keyword | molecular epidemiology | en |
dc.subject.keyword | Enterobacterales | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM//LX22NPO5103 | |
dc.date.embargoStartDate | 2023-10-02 | |
dc.date.embargoEndDate | 2023-06-26 | |
dc.type.obd | 110 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.obd | 622451 | |
dc.identifier.riv | RIV/00216208:11140/22:10454791 | |
dc.subject.rivPrimary | 30000::30300::30303 | |
dcterms.isPartOf.name | Czech Chemical Society Symposium Series | |
dcterms.isPartOf.issn | 2336-7202 | |
dcterms.isPartOf.journalYear | 2022 | |
dcterms.isPartOf.journalVolume | 20 | |
dcterms.isPartOf.journalIssue | 6 | |
uk.faculty.primaryId | 111 | |
uk.faculty.primaryName | Lékařská fakulta v Plzni | cs |
uk.faculty.primaryName | Faculty of Medicine in Pilsen | en |
uk.faculty.secondaryId | 110 | |
uk.faculty.secondaryName | 3. lékařská fakulta | cs |
uk.faculty.secondaryName | Third Faculty of Medicine | en |
uk.department.primaryId | 100012968318 | |
uk.department.primaryName | Biomedicínské centrum | cs |
uk.department.primaryName | Biomedical Center | en |
uk.department.secondaryId | 100033743623 | |
uk.department.secondaryId | 1359 | |
uk.department.secondaryName | Ústav mikrobiologie 3. LF UK, FNKV a SZÚ | cs |
uk.department.secondaryName | Department of Microbiology 3FM CU, UHKV and NIPH | en |
uk.department.secondaryName | Ústav mikrobiologie | cs |
uk.department.secondaryName | Department of Microbiology | en |
uk.event.name | The first annual meeting of the National Institute of Virology and Bacteriology | |
dc.description.pageRange | 401-402 | |
dc.type.obdHierarchyCs | ABSTRAKT::abstrakt::abstrakt v konferenčním sborníku | cs |
dc.type.obdHierarchyEn | ABSTRACT::abstract::abstract in conference proceedings | en |
dc.type.obdHierarchyCode | 110::130::462 | en |
uk.displayTitle | Epidemic spread of KPC-producing bacteria in the Czech Republic | en |