dc.contributor.author | Mašek, Tomáš | |
dc.contributor.author | del Lano, Edgar | |
dc.contributor.author | Sharma, Khushboo | |
dc.contributor.author | Gahurová, Lenka | |
dc.contributor.author | Dvořan, Michal | |
dc.contributor.author | Rajan, Iappan | |
dc.contributor.author | Aleshkina, Daria | |
dc.contributor.author | Končinská, Markéta | |
dc.contributor.author | Bora, Pablo | |
dc.contributor.author | Roučová, Kristina | |
dc.contributor.author | Lin, Chih-Jen | |
dc.contributor.author | Bruce, Alexander William | |
dc.contributor.author | Kubelka, Michal | |
dc.contributor.author | Šušor, Andrej | |
dc.contributor.author | Pospíšek, Martin | |
dc.date.accessioned | 2023-08-02T12:10:21Z | |
dc.date.available | 2023-08-02T12:10:21Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14178/1992 | |
dc.description.abstract | Polysome profiling has provided a gold-standard method to precisely assay functional genome readout at a given time point. The wide-spread utilisation of polysome profiling has nevertheless been historically hindered by its intrinsic complexity, time-consuming nature, limited capacity for high throughput adaptation and its requirement for relatively large initial sample sizes. Here, we present Scarce Sample Polysome Profiling (SSP-profiling); method that demonstrates a combination of the sucrose gradient ultracentrifugation in small SW55Ti tubes with the qRT-PCR-based quantification of 18S and 28S rRNAs in fractionated polysome profile. SSP-profiling is suitable for both scarce and conventional sample sizes and is compatible with downstream RNA-seq to identify polysome associated transcripts | en |
dc.language.iso | en | |
dc.rights | Creative Commons Uveďte původ 4.0 International | cs |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.title | Polysome profiling of oocytes, early development, and cell cycle in mammals | en |
dcterms.accessRights | openAccess | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/legalcode | |
dc.date.updated | 2024-02-18T20:40:36Z | |
dc.subject.keyword | Polysome | en |
dc.subject.keyword | profiling | en |
dc.subject.keyword | SSP-profiling | en |
dc.subject.keyword | RNA | en |
dc.relation.fundingReference | info:eu-repo/grantAgreement/MSM//LX22NPO5103 | |
dc.date.embargoStartDate | 2024-02-18 | |
dc.contributor.organizer | RNA Society | |
dc.type.obd | 110 | |
dc.type.version | info:eu-repo/semantics/submittedVersion | |
dc.identifier.obd | 633850 | |
dc.subject.rivPrimary | 10000::10600 | |
dcterms.isPartOf.name | Neuvedeno | |
dcterms.isPartOf.journalYear | 2023 | |
dcterms.isPartOf.journalVolume | 2023 | |
dcterms.isPartOf.journalIssue | Neuvedeno | |
uk.faculty.primaryId | 115 | |
uk.faculty.primaryName | Přírodovědecká fakulta | cs |
uk.faculty.primaryName | Faculty of Science | en |
uk.department.primaryId | 1034 | |
uk.department.primaryName | Katedra genetiky a mikrobiologie | cs |
uk.department.primaryName | Department of Genetics and Microbiology | en |
uk.event.name | The 28th Annual Meeting of the RNA Society | |
dc.description.pageRange | nestránkováno | |
dc.type.obdHierarchyCs | ABSTRAKT::abstrakt::abstrakt v elektronické podobě | cs |
dc.type.obdHierarchyEn | ABSTRACT::abstract::abstract in e-form | en |
dc.type.obdHierarchyCode | 110::130::136 | en |
uk.displayTitle | Polysome profiling of oocytes, early development, and cell cycle in mammals | en |